GC-MS analysis for Structural Identification and Bioactive Compounds in Methanolic Leaf Extract of Mallotus oppositifolius

Abstract

The aim of the present study is to investigate the bioactive compounds from the leaf extract of Mallotus oppositifolius using GCMS analysis. The chromatogram showed nine peaks indicating the presence of nine compounds in the extract. The major phytocompounds in the leaf were Glutaconic anhydride with the highest concentration in the extract, 40.19 peak area %, RT 22.686 and molecular formula C₅H₄O₃;  2-Mercaptophenol with  18.23 peak area %, RT 22.068 and molecular formula C₆H₆ OS. Iso-Valreic and Valeric acids with 12.39,2.53 peak area %, RT 3.676,7.037 and the same molecular formula C₅H₁₀ O₂ which had been proposed to have anticonvulsant effect in valerian and act as neurotransmitter. Oleamide with the least concentration of 2.15 peak area %, RT 27.959 and molecular formula C₁₈H₃₅NO which could induce sleep in animals, being studied as a potential medical remedy for mood and sleep disorder and cannabinoid–regulated disorder. The phytochemicals in Mallotus oppositifolius could be of therapeutic importance.

Keywords

• GCMS analysis